Education:

BS (1990), Xiamen University 

PhD (1996),  Xiamen University

Principal Research Interests:
Development of advanced instrumentation and methodologies for biochemical analysis and biosensing. The main research focuses include ： 1) Development of high sensitivity flow cytometry (HSFCM) to address the challenge of quantitative physical and      biochemical characterization of single nanoparticles in nanotechnology, nanobiotechnology, nanomedicine,  biopharmaceuticals, and basic science. 2) High-throughput, high-resolution, and multiparameter characterization of single natural and synthetic nanoparticles such as gold, silver, silica, polymeric, and iron oxide nanoparticles, lipid-based nanomedicines, quantum dots, and viruses. 3) Specific analysis of individual bacteria for pathogen identification, antibiotic resistance detection, and biological function studies. 4) Development of advanced methodologies for the individual analysis of subcellular organelles such as mitochondria, extracellular vesicles (microvesicles and exosomes), and chromosomes. 5) Development of microsphere-based suspension array technology for biomedical and biochemical applications.

Selected Recent Publications:

1. 1. Chen, CX; Zhou, YX; Chen, C; Zhu, SB; Yan, XM*; Quantification of Available Ligand Density on the Surface of Targeted Liposomal Nanomedicines at the Single-Particle Level, ACS Nano 2022, 16, 6886-6897.

2. 2. Liu, HS; Tian, Y; Xue, CF; Niu, Q; Chen, C; Yan, XM*; Analysis of Extracellular Vesicle DNA at the Single-Vesicle Level by Nano-flow cytometry, J. Extracell. Vesicles 2022, 11, e12206.

3. 3. Niu, Q;^# Ma, L;^# Zhu, SB; Li, L; Zheng, QS; Hou, JB; Lian, H; Wu, LN; Yan, XM*; Quantitative Assessment of the Physical Virus Titer and Purity by Ultrasensitive Flow Virometry, Angew. Chem. Int. Ed. 2021, 60, 9351-9356.

4. 4. Chen, CX;* Sun, MD; Wang JL; Su, LY; Lin, JJ; Yan, XM*; Active Cargo Loading into Extracellular Vesicles: Highlights the Heterogeneous Encapsulation Behavior, J. Extracell. Vesicles 2021, 10, e12163.

5. 5. Gao, KM;^# Zhou, YX;^# Lu, QY; Lu, JY; Su, LY; Su, R; Zhang, MM; Tian, Y; Wu, LN; Yan, XM*; High-Throughput Human Telomere Length Analysis at the Single-Chromosome Level by FISH Coupled with Nano-Flow Cytometry. Anal. Chem. 2021, 93, 9531-9540.

6. 6. Tian, Y; Gong, MF; Hu, YY; Liu, HS; Zhang, WQ; Zhang MM; Hu, XX, Aubert, D; Zhu, SB; Wu, LN; Yan, XM*; Quality and Efficiency Assessment of Six Extracellular Vesicle Isolation Methods by Nano-Flow Cytometry, J. Extracell. Vesicles 2020, 9, 1697028 (ESI高被引论文)

7. 7. He, SB; Hong, XY; Zhang, MM; Wu, LN; Yan, XM*; Label-Free Detection of Bacteria in Fruit Juice by Nano-Flow Cytometry, Anal. Chem. 2020, 92, 2393-2400.

8. 8. Lian, H; He, SB; Chen, CX; Yan, XM*; Flow Cytometric Analysis of Nanoscale Biological Particles and Organelles, Annu. Rev. Anal. Chem. 2019, 12, 389-409.

9. 9. Tian, Y; Ma, L; Gong, MF; Su, GQ*, Zhu, SB; Zhang, WQ; Wang, S; Li ZB; Chen CX; Li, LH; Wu, LN; Yan, XM*; Protein Profiling and Sizing of Extracellular Vesicles from Colorectal Cancer Patients via Flow Cytometry, ACS Nano 2018, 12, 671-680 (ESI高被引论文)

10. 10. Zhang, WQ; Tian, Y; Hu, XX; He, SB; Niu, Q; Chen, CX; Zhu, SB; Yan, XM*; Light-Scattering Sizing of Single Submicron Particles by High-Sensitivity Flow Cytometry, Anal. Chem. 2018, 90, 12768-12775.

11. 11. Wang, S; Li, LH; Jin, SH; Li, WF; Hang, W; Yan, XM*; Rapid and Quantitative Measurement of Single Quantum Dots in a Sheath Flow Cuvette, Anal. Chem. 2017, 89, 9857-9867.

12. 12. Wu, LN; Wang, X; Zhang, JQ; Luan, T; Bouveret, E.; Yan, XM*; Flow Cytometric Single-Cell Analysis Enables Quantitative in vivo Detection of Protein-Protein Interactions via Relative Reporter Protein Expression Measurement, Anal. Chem. 2017, 89, 2782-2789.

13. 13. Chen, CX; Zhu, SB; Wang, S; Zhang, WQ; Cheng, Y; Yan, XM*; Multiparameter Quantification of Liposomal Nanomedicines at the Single-particle Level by High Sensitivity Flow Cytometry, ACS Appl. Mater. Interfaces 2017, 9, 13913-13919.

14. 14. Ma, L^#; Zhu, SB^#; Tian, Y; Zhang, WQ; Wang, S; Chen, CX; Wu, LN; Yan, XM*; A Label-Free Analysis of Single Viruses with a Resolution Comparable to That of Electron Microscopy and the Throughput of Flow Cytometry, Angew. Chem. Int. Ed. 2016, 55, 10239 –10243. (Hot Paper)

15. 15. Zhu, SB; Ma, L; Wang, S; Chen, CX; Zhang, WQ; Yang, LL; Hang, W; Nolan, J. P.; Wu, LN; Yan, XM*; Light-Scattering Detection below the Level of Single Fluorescent Molecules for High-Resolution Characterization of Functional Nanoparticles. ACS Nano 2014, 8, 10998-11006.